Chitosan Lactate

Catalog	ACM9012764-43
CAS	9012-76-4
Description	High DDA% – Low Mw (Water Soluble)
Molecular Formula	(C6H11NO4)n
Appearance	Light yellow, free flowing powder
Application	Biocompatible, antibacterial and environmentally friendly polyelectrolyte with a variety of applications including water treatment, chromatography, additives for cosmetics, textile treatment for antimicrobial activity, novel fibers for textiles, photographic papers, biodegradable films, biomedical devices, and microcapsule implants for controlled release in drug delivery.
Storage	<25°C, cool dry environment, well-sealed
Content	Lactate content 42%
Feature	Degree of Deacetylation (DDA): ≤ 95.0%
Form	Powder
Moisture Content	0.0805
Packaging	100g, 250g
pH	4.21
Ignition Residue	0.3
Type	Crustacean Source
Viscosity	< 200 (mpa.s, 20 °C)

Case Study

Combined Antitumor Effect of Chitosan Lactate Nanoparticles Loaded with CD73-Specific siRNA

Jadidi-Niaragh F, et al. Journal of Controlled Release, 2017, 246, 46-59.

CD73-specific siRNA-loaded chitosan-lactate nanoparticles (ChLa NPs) were prepared and tested for the efficacy when used together with tumor lysate pulsed dendritic cells (DCs) vaccine in 4T1 (murine) breast cancer mice. The data demonstrated that intravenous delivery of CD73-specific siRNA-loaded NPs suppressed CD73 levels in tumor cells and lowered the growth and spread of tumors and improved mice's survival. Moreover, DC vaccine combined with CD73 siRNA-loaded nanocarriers can boost the antitumor immune system in tandem.
Preparation of siRNA loaded ChLa NPs
ChLa nanoparticles (NPs) were created through the ionic gelation of ChLa using a solution of siRNA and TPP. In brief, 20 µl of siRNA (equivalent to 90 µg) in DEPC-treated water was combined with an 800 µl TPP solution (0.7 mg/mL) and then added dropwise to a 1 mL ChLa solution (5.5 mg/mL) while stirring magnetically. The NPs formed after incubating in the dark for 30 minutes at room temperature, and the formation of the siRNA-NP complex was verified via 2% agarose gel electrophoresis.
The ChLa NPs exhibited high siRNA encapsulation efficiency, ensuring complete binding with siRNA and providing protection against degradation by serum and heparin. The transfection rate was measured at 53.6%, effectively reducing CD73 expression in 4T1 cells. Furthermore, analysis revealed that 4T1 cells treated with the CD73-specific siRNA-loaded NPs displayed CD73 levels on their surface that were 16 times lower compared to untreated cells.

Chitosan Lactate Blended Films with Oxidized Starch or Gelatin and Their Antimicrobial Properties

Kowalczyk D, et al. International Journal of Biological Macromolecules, 2015, 77, 350-359.

A series of films based on chitosan lactate (CHL) with oxidized potato starch (OPS) or gelatin (GEL) were prepared and their antimicrobial properties were investigated.
· Antimicrobial Properties
CHL was effective against target organisms including Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Bacillus carotovora and Escherichia coli) except Rhizopus nigricans. Moreover, the films prepared by replacing half of CHL with OPS or GEL showed significantly improved mechanical strength and reduced permeability compared to pure CHL films. CHL film was the only film that showed antimicrobial efficacy. Antifungal activity of CHL and CHL50/GEL50 films against Alternaria alternata and Pseudomonas sclerotiorum was detected.
· Film preparation
The films were formulated from 5% (w/w) water-based biopolymer solutions with 3% (w/w) sorbitol. The 80-95% deacetylated chitosan lactate (CHL) was dissolved in sorbitol and distilled water and let it heat for 30 minutes in a 90°C water bath stirring constantly. The film-forming solutions (FFSs) were 50:50 mixtures of CHL/OPS or CHL/GEL, and FFSs that did not include CHL were also made from OPS and GEL.
Following cooling to 50 °C, the pH of the FFS was measured with a pH meter. The FFSs were then poured onto flat polycarbonate trays (12 x 12 cm) and dried at approximately 50% relative humidity (RH) and 25°C for about 24 hours. To ensure consistent film thickness, a uniform amount of 0.011 g of total solids was applied to a 1 cm² area of the tray.