Chitin from shrimp shells, BioReagent

Catalog	ACM1398614-7
CAS	1398-61-4
Structure
Description	suitable for analysis of chitinase
Synonyms	(1,4)-N-acetyl-D-glucos-2-amine
Canonical SMILES	CC(=O)N[C@@H]1[C@H](O)O[C@@H](CO)[C@H](O)[C@H]1O
InChI	1S/C8H15NO6/c1-3(11)9-5-7(13)6(12)4(2-10)15-8(5)14/h4-8,10,12-14H,2H2,1H3,(H,9,11)/t4?,5?,6?,7?,8-/m1/s1
InChI Key	OVRNDRQMDRJTHS-WTZNIHQSSA-N
Application	Chitin from shrimp shells, a long-chain polymer of a β-1,4 linked N-acetylglucosamine residues, may be used to study its physicochemical properties and role in the formation of arthropod exoskeletons. It may be used to identify and characterize chitinase(s) and chitin deacetylase(s), as a standard to study the structure of chitins from other species and as a viscosity/thickening agent or binder. Chitin from shrimp shells may be used in chitin asthma and allergy research.
Form	purified powder
Grade	BioReagent
MDL Number	MFCD00466914
Packaging	250 mg in poly bottle 1, 5 g in poly bottle
Type	biological source: shrimp (Pandalus borealis)

Case Study

Chitosan Nanofiber Paper for Optical Biosensing

Naghdi, Tina, et al. ACS applied materials & interfaces, 2020, 12(13), 15538-15552.

By embedding/fixing various nanoparticles in the 3D nanonetwork scaffold of chitosan nanofiber (ChNF) paper, a series of transparent, efficient, biocompatible, flexible and miniaturized optical sensing bioplatforms can be realized. The nanoparticles explored in this work include plasmonic nanoparticles (silver and gold nanoparticles), photoluminescent nanoparticles (CdTe quantum dots, carbon dots and NaYF₄:Yb³⁺@Er³⁺&SiO₂ upconversion nanoparticles) and colorimetric reagents (curcumin, disulfide hydrazone, etc.).
Production of ChNF paper
Shrimp shells (Littopenaeus vannamei) were initially treated with hydrochloric acid to remove minerals and then refluxed with sodium hydroxide twice to eliminate proteins. In the second alkaline treatment, hydrogen peroxide (0.1 wt%) was used to remove pigments. The resulting white shells were ground into chitin powder using a chicken grinder. This powder was then converted into a 1 wt% chitin suspension and passed through a super disk grinder for mechanical nanofibrillation. The resulting chitin suspension was transformed into a highly viscous wet gel of chitin nanofibrils (ChNF). A well-dispersed ChNF suspension (0.2 wt%) was filtered under vacuum and subsequently hot-pressed at 100 °C and 2 MPa for 1 hour. This process produced transparent ChNF paper with a grammage of 60±3 g/m2, a density of 1.1 g/cm3, and a thickness of approximately 45 µm.

Chitin/Silk Fibroin/TiO2 Bionanocomposite for Use as Wound Dressing

Mehrabani, Mojtaba Ghanbari, et al. International journal of biological macromolecules, 2018, 116, 966-976.

A chitin/silk fibroin/TiO2 nanocomposite was synthesized by freeze drying method for use as wound dressing, which is interconnected microporous biodegradable and biocompatible with high antibacterial, coagulant and mechanical strength properties.
Preparation procedure of Chitin/silk fibroin/TiO2 dressing
· A 2.5 wt% chitin solution was prepared by dissolving purified chitin powder in a 11 wt% NaOH and 4 wt% urea solvent using a freeze-thaw method. An equal volume of a 2.5 wt% silk fibroin solution was added to the chitin solution to create a 50:50 v/v% chitin/silk fibroin solution. Glycerol was added as a plasticizer (50 wt%, based on the combined weight of chitin and silk fibroin). The solution was mixed for 12 hours at room temperature.
· Predetermined amounts of TiO2 nanoparticles were dispersed in distilled water using a probe-type sonicator and added to the chitin/silk fibroin solution to prepare nanocomposites containing 0.5, 1.5, and 3.0 wt% TiO2 (denoted as T0.5, T1.5, and T3.0, respectively).
· The solution was mixed for an additional 24 hours and then crosslinked with 0.25% (v/v) glutaraldehyde in a 1:32 ratio over 5 hours. The resulting paste was frozen at -20 °C for 12 h and freeze dried at -70 °C for 48 h to obtain 3D nanocomposite scaffolds as wound dressing bandages.